merged

Isolation and Identification of Hydrocarbon- Degrading Bacteria from
Polychaete
Nurul Fadhilah Binti Mohd Nor
Supervisor: Dr. Fazilah Binti Ariffin
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Petroleum are used as a primary source of energy for industries and transportation in
daily life that caused pollution especially for sea. The studies have shown that
polychaete can affect the composition of bacterial communities in oil-contaminated
sediments. The primary role of polychaete in bioremediation is to increase the
solubility and bioavailability of hydrocarbons by oil-degrading bacteria. Therefore, the
aim of this study was to isolate, screen, and identify hydrocarbon-degrading bacteria
that present in the polychaete Marphysa moribidii of oil-polluted waters in Pantai
Morib, Selangor. The bacteria was isolated from gut fluid using spread plate method
and identified based on morphology, biochemical test and BBL Crystal Identification
Kit. As a result, two bacteria were tentatively identified such as Gardnerella vaginalis
and Acinetobacter iwoffi showed positive effect. Based on the result, it can be
concluded that the hydrocarbon-degrading bacteria that present in the polychaete
are beneficial to improve the quality of the water and hence, the combination of these
isolated bacteria could be a good bioremediation agent for sea water.
370 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018