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Assesment of Acrylamide Compound Cytotoxicity Mechanism in Human
Colon Cell Line (Hct 116) Via Mapk Pathway
Nur Syazwina Binti Abdul Manaf
Supervisor: Dr. Nurul Huda Binti Abdul Kadir
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Acrylamide occur if food, rich in salt and starch content were exposed to high
temperatures exceed 120 ºC during foods processing. Development of colon cancer
was believed due to consumption of foods contain Acrylamide. Cytotoxicity effects of
Acrylamide were evaluated at various concentrations using AlamarBlue Assay on HCT
116 cells. Acrylamide were potentially reduce viability of HCT 116 cells with exclusive
50% inhibition concentration (IC50) on each time point of the treatments (IC50:
20.0mM, 25.1mM, and 28.2mM after 24h, 48h, and 72h). This cytotoxicity activity
was corresponded with the ROS activity where the treated HCT 116 cells shown criteria
of apoptosis. ERK 1/2, p-ERK, Bcl-2 and Caspase-3 mitogen-activated protein kinase
(MAPK) was detected using Western blot technique. Our result shown that protein
expressions of treated HCT 116 with Acrylamide was upregulated and down regulated
after 48 hour, suggesting that cell death event induced by Acrylamide was due to
apoptosis.
389 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018