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Screening of Xanthine Oxidase and Cyclooxygenase-2 Inhibitory
Activities in R-38 Herbal Medicine
Ng Hui Ci
Supervisor: Prof. Dr. Fadzilah Adibah Abd Majid
Co-supervisor: Dr. Thilahgavani A/P Nagappan
Bachelor of Applied Science
(Biodiversity Conservation and Management)
School of Marine and Environmental Sciences
Uric acid and prostaglandins are among the important factors that induce gout.
Xanthine oxidase is an enzyme which catalyses purine bases into uric acid, while
cyclooxygenase catalyses arachidonic acid to form prostaglandins. R-38 herbal
medicine is made up of five different local herbs which aimed to treat gout. In
order to investigate, the current study was carried out to determine whether
acetone, ethanol, petroleum ether and water extracts of R-38 herbal medicine
show inhibition activity of xanthine oxidase and cyclooxygenase-2 in vitro. The
determination of the inhibition activity of XO and COX-2 were carried out using
XO and COX-2 bioassays. For XO and COX-2 assays, all the four extracts of R-
38 (acetone, ethanol, petroleum ether, and water) revealed their own inhibition
activities compare to positive control. The acetone extract of R-38 shown the
highest inhibition level for both assays. It can be suggested that R-38 possess
therapeutic activities against gout.
1717 | UMT UNDERGRADUATE RESEARCH DAY 2018