merged

Effect Of Carbon Sources In The Biosynthesis Of Poly(3-Hydroxybutyrate)
Using Novosphingobium Panipatense Umtkb-4
Sarenyah A/P Manimaran
Supervisor: Dr.Vigneswari Sevakumaran
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Petroleum-based plastics are a threat to the ecosystem due to its non-biodegradable
properties. Interestingly, poly(3-hydroxybutyrate) [P3HB] a microbial derived
biodegradable biopolymer has proven as a promising candidate to combat this
environmental damage. In this study, biosynthesis of P3HB batch fermentation is
conducted using inexpensive carbon substrates such as glucose, fructose, sucrose and
glycerol using locally isolate bacterium, Novosphingobium panipatense under nitrogen
limiting condition. Based on the findings, cultivation at C/N ratio 20 and 30 using
glycerol as the sole carbon source has accumulated the highest P3HB up to 11.99 ±
0.91 wt% and 13.54 ± 1.18 wt% with the 0.50g/L and 0.51g/L dry cell weight (DCW)
respectively. Therefore, it is proven that N. panipatense UMTKB-4 is capable of
producing P3HB with component of by-product as the sole carbon source. Thus, more
value-added green material can be produced from the conversion of agro-industrial
by-products which are abundant in Malaysia.
400 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018