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Isolation and Identification of Pathogenic Bacteria from Quarantine Tanks
of Hippocampus spp.
Nur Haniza binti Misbah
Supervisor: Prof Dr. Mohd. Effendy Bin Abd. Wahid
Bachelor of Applied Sciences (Fisheries)
School of Fisheries and Aquaculture Sciences
The death of seahorses in quarantine tank at Akuatrop Hatchery has led to the
isolation and identification of pathogen from the remaining alive seahorses. The
isolation was done employing Marine Nutrient Agar (MNA) and pure culture was
obtained to identify the bacteria by VITEK® 2 Systems located at Institute of Marine
Biotechnology (IMB), Universiti Malaysia Terengganu. Samples were collected from
tail, dorsal, pouch and stomach from unhealthy and dead seahorses. Results showed
that (88%) of the pathogen isolated are Vibrio spp., (3%) are from Aeromonas
salmonicida, (3%) of Myroides spp., (3%) of Serratia ficaria and (3%) of
Sphingomonas paucimobilis. Further identification on Vibrio spp. were used by
Thiosulfate Citrate Bile Salts Sucrose (TCBS) agars .Vibrio parahaemolyticus produced
light bluish colonies and Vibrio alginolyticus produced large and yellow colonies on the
TCBS agar. The study of the role of pathogenic bacteria in disease development of
seahorses play important role to manage seahorses cultivation and development in
hatchery.
624 | UMT UNDERGRADUATE RESEARCH DAY 2018