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Histopathological changes in cultured shrimp, Litopenaeus vannamei<br />

infected by Enterocytozoon hepatopenaei sp.<br />

(Microsporidia: Enterocytozoonidae)<br />

Nur Hidayah Binti Md Nor<br />

Supervisor: Assoc. Prof Dr Marina Binti Hassan<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

The microsporidian, Enterocytozoon hepatopenaei (EHP) is an intracellular parasite<br />

that has become a critical threat to the shrimp farming, Litopenaeus vannamei in<br />

Southeast Asia. This parasite replicate in the hepatopancrease and midgut, and<br />

infected shrimp exhibit reduce feeding and severely retarded growth with variation of<br />

size. In this study, hepatopancrease sample from 30 individual of shrimp L. vannamei<br />

from Terengganu area that suspected infected with EHP disease were analyzed by<br />

using histology technique. The results showed that all the shrimp examined were<br />

infected with EHP. Infected shrimp showing basophilic inclusions in hepatopancrease<br />

tubule epithelial cells. Midgut epithelial cells were also infected. All the samples<br />

showed the presence of microsporidian spores in cytoplasm of hepatopancreatic<br />

tubule epithelial cells.<br />

515 | UMT UNDERGRADUATE RESEARCH DAY 2018

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