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The Cytotoxicity Effect and the Mode of Cell Death of Acanthaster Planci<br />

Extract on Human Cervical Cancer Cell Line (HELA)<br />

Nur Shahida binti Ahmad Sofian<br />

Supervisor: Dr. Vigneswari Sevakumaran<br />

Co-Supervisor: Prof Dr. Tengku Sifzizul Tengku Muhammad<br />

Bachelor of Science (Biological Sciences)<br />

School in Fundamental Science<br />

Cervical cancer is common devastating disease among female worldwide and current<br />

treatment cause various side effect. Researcher has leverage the effort on finding new<br />

effective anti-cancer drug. A.planci are venomous starfish with active metabolites that<br />

posess pharmacology activity. This studies are designated in vitro studies to asses<br />

cytotoxicity and apoptotic effect of A.planci extract againts human cervical cancer cell<br />

(HeLa) via MTS assay and Annexin V/Propadium iodide assay respectively. MTS assay<br />

shows cell viability was inhibited by the A.planci extract (IC 50 = 52.48 µg/ml). Thus,<br />

A.planci methanolic extract shows anti-proliferative effect but not anti-cancer<br />

properties. The induction of apoptosis confirmed by Annexin V-FITC/PI double staining<br />

due to the translocation of PS (phosphatidylserine) on the cell surface. The<br />

morphological observation shows HeLa cell with early apoptotic feature emited green<br />

fluorescence while late apoptotic cells emited red fluorescence.This results revealed<br />

that A. planci extract posses low cytotoxicity and apoptotic effect on HeLa cell<br />

402 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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