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The Study of Xanthine Oxidase Inhibitory Activity of Flavonoid Compound
from Different Parts of Andrographis Peniculata
Arifah Radhiah Binti Mohd Arif
Supervisor: En Mohamad Hussin Bin Haji Zain
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Xanthine Oxidase (XO) is an enzyme known to contribute to the production of uric
acid in human body. Flavonoid, one of the natural sources of Xanthine Oxidase
Inhibitor (XOI) was extracted from leaves, stems and roots of Andrographis Peniculata
by using solvent extracted method. In this research, the presence of flavonoid
compound in extracted samples was identified using phytochemical test and
characterized by FTIR Spectroscopy. The highest total flavonoid content was in stem
(0.098mg/ml), followed by leave (0.074mg/ml) and root (0.073mg/ml).This was then
proven with the maximum inhibition rate of XO assay was in stem (89.4%), followed
by leave (69.5%) and root (68.2%). However Allopurinol gave have the highest
inhibition rate at (99.9). According to IC50 evaluation showed Allopurinol had lower
IC50 value (0.002mg/ml) as compared to stem (0.015mg/ml).The inhibition of XO
assay in stem was 10.5% less than Allopurinol. Thus stem of Andrographis Peniculata
has high potential to be used in gout treatment.
473 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018