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Isolation and Characterization of Exopolysaccharides (Eps)-Producer<br />

Bacteria from Marphysa Moribidii<br />

Siti Hazwani Binti Rosdi<br />

Supervisor: Dr. Azila binti Adnan<br />

Bachelor of Science (Biological Science)<br />

School of Fundamental Science<br />

Exopolysaccharide (EPS) is the natural high molecular weight polymer that usually<br />

produces by bacteria for their protective mechanism and survival. Lactic acid bacteria<br />

are the common bacteria that have been extensively studied on EPS production. EPS<br />

is important because they can be an alternative class of bio thickeners that are widely<br />

used in food industry. In this project, the bacteria, which isolated from the polychaete<br />

Marphysa moribidii are used to prove the ability to produce EPS. The aim of this study<br />

is to characterize and identify the isolated bacteria from the gut of Marphysa moribidii<br />

and prove its ability to produce EPS. For the identification of the bacteria, several<br />

biochemical tests were conducted. It is found that the bacteria isolated come from<br />

family Bacillaceae, which is Bacillus cereus. Total carbohydrate analysis were also<br />

carried out to identify the production of EPS by Bacillus cereus. In conclusion,<br />

Marphysa moribidii has the EPS-producer bacteria, which are able to produce EPS.<br />

Further study will be a focus on the modification of the EPS produced by this bacteria,<br />

which is Bacillus cereus that will give more benefit to the industry.<br />

355 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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