merged

Growth and Lipid Production of Microalgae Chlorella Vulgaris Grown Under
Nitrogen Limited Condition
Izhar Adib bin Zainal Anuar
Supervisor: Dr. Malinna binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Microalgae are unicellular eukaryotic organisms which can reproduce rapidly and serve
as an alternative way to gain energy sources. Microalgae have been worldwide studies
but the growth and lipid body formation of microalga C. vulgaris grown under nitrogen
limitation is not widely studied. This study was conducted to observe the growth and
lipid body formation in C. vulgaris. Microalga C. vulgaris was cultured in two different
conditions which are nitrogen control and limiting. The growth of C. vulgaris was
counted for every two days using haemocytometer. Lipid body formation for both
conditions was observed for every seven days using Nile Red staining. The highest
number of cell in treatment condition was found at DAT-18. Nile Red staining
penetrated into the microalgae and stained lipid body. It is found that DAT-21 in the
treatment condition produced more lipid body. Therefore, further study was needed
for extracting the lipid for future used.
350 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018