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Non-Enzymatic Antioxidants of Chlorella Vulgaris in Different Growth
Phases
Suhaila binti Azizam
Supervisor: Dr. Norhayati binti Yusuf
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Chlorella vulgaris is a unicellular microorganism that offers health benefits due to the
concentrated antioxidants produced. They also have efficient scavenging system for
reactive oxygen species (ROS) which to protect from destructive oxidative reactions.
This study was conducted to determine and compare the amount of α-Tocopherol,
ascorbic acid, and carotenoids at different growth phases in C. vulgaris. Microalgae
was culture in F/2 medium with batch culture system. The growth curve was analysed
through cell counting, whereas the content of the non-enzymatic antioxidants were
measured using UV spectrophotometer. α-Tocopherol production was higher at the
stationary phase (97.38±4.18 µg/g.fwt), whereas the higher production of ascorbic
acid (266.67 ± 22.22 µg/g. fwt) and carotenoid (8.16 ± 2.52 µg/g. fwt) are at decline
phase. Above results indicated that all the five phases have different amount of nonenzymatic
antioxidants. The higher amount of non- enzymatic antioxidants are at
decline and stationary phases. Further study is required for development of the
medium with proper of nitrogen and phosphorus ratio.
329 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018