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Fatty Acid Profiling of Namalycastis Sp. (Polychaete) from Setiu Wetland
and Their Potential against Mcf-7 Cell
Choong Yau Kuan
Supervisor: Dr. Tuan Nurul Sabiqah binti Tuan Anuar
Bachelor of Science (Analytical and Environmental Chemistry)
School of Marine and Environmental Sciences
Universiti Malaysia Terengganu
This study was undertaken to determine the fatty acid profile of a new record of
polychaete (Namalycastis sp.) from mangrove areas in Setiu Wetland and their
potential against MCF-7 cell (breast cancer cell). Bligh and Dyer method was used to
extract lipid contents from the polychaete and its profile was analyzed by using Gas
Chromatography Flame Ionization Detector (GC-FID). Tumor MCF-7 cell inhibition
analysis was performed using In-vitro MTT test to determine the IC50 level using
different concentration of lipids (1000 µg/mL to 0.2 µg/mL). The fatty acid composition
in polychaete showed highest amount of saturated fatty acids (SFA; 65.67%), followed
by monosaturated fatty acids (MUFA; 21.79%) and polyunsaturated fatty acids (PUFA;
12.55%). Dietary intake is greatly affect the lipid composition of the polychaete. In
antitumor testing, it showed that some MCF-7 cell viability decreases as the
concentration of lipids increases. Polychaete are played important roles in aquaculture
and prevent cancer cells due to its nutritional values.
1940 | UMT UNDERGRADUATE RESEARCH DAY 2018