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Sperm Activation and Motility of Molly, Poecilia Sphenops<br />

Muhammad Azzim Bin Omar<br />

Supervisor: Dr Lokman Norhakim Bin Norazmi<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

Poecilia sphenops is live-bearing species. This species undergoes internal fertilization<br />

instead of laying eggs. The objectives of this study are to investigate the sperm<br />

activation and motility of molly, P. sphenops on different solutions. The solution that<br />

used in this study are fresh water (H2O), saline water at 9ppt and Hanks’ balanced<br />

salt solution (HBSS) at 300 mOsmol/kg. The molly was dissect and removed the testis.<br />

The testis was place on slide glass and put the solution with ratio 1:100. Then the<br />

slide glass was observe under compound microscope. The estimation of sperm<br />

activation and motility was record. The saline water at 9ppt causing the most<br />

activation and motility of sperm P.sphenops than freshwater and Hanks’ balanced salt<br />

solution (HBSS). It is because the saline water at 9ppt will promote the reproduction<br />

hormone to activation and motility of sperm molly P.sphenops. So, the breeder will<br />

know the best solution to make theirs production became higher.<br />

548 | UMT UNDERGRADUATE RESEARCH DAY 2018

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