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Molecular Detection on Tilapia Lake Virus (TiLV) In Black Tilapia
(Oreochromis Niloticus)
Vickran Pillai S/O Mohan
Supervisor: Dr Sandra Catherine Zainathan
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture
This study was conducted to detect the presence or absence of Tilapia Lake Virus
(TiLV) in black tilapia (Oreochromis niloticus). About fifty samples was collected for
this study from Pusat Pembangunan Akuakultur, Jitra. This study was conducted as
Malaysia is among the high-risk country listed to be affected by TiLV. One step RT-
PCR method and Nested RT-PCR method was used for detection of TiLV in this study.
The average weight of all tilapia samples was 3.13g and average length of all tilapia
samples was 4.87cm. There were seven tilapia samples that showed growth
abnormality of caudal fin and wound on body, which is sample S1, S10, S12, S16, S24,
S25 and S48. These clinical signs is not related to infection of TiLV. The one step RT-
PCR and nested RT-PCR results showed that all the samples generated negative results
for the presence of TiLV. The absence of TiLV-specific band showed that the fish were
free from TiLV infections, which may indicate that the fish were in good condition. In
conclusion, TiLV were absent in the tilapia samples.
575 | UMT UNDERGRADUATE RESEARCH DAY 2018