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Effect of Various Shape Synthesized Silver Nanoparticles as an
Antimicrobial Agent
Nurul Hidayah Binti Mohd Azmi
Supervisor: Dr Hanis Binti Mohd Yusoff
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Silver nanoparticles (Ag-NPs) of different shape were prepared by chemical reduction
method. Scanning electron microscopy (SEM), UV-visible spectroscopy (UV-VIS), and
X-ray diffraction (XRD) techniques were used to characterize the prepared Ag-NPs.
SEM micrographs showed three different shapes of Ag-NPs which are spherical,
triangular and flower like with size range 200 to 300 nm, 100 to 300 nm and 1690 nm
respectively. The optical characteristics of different shape Ag-NPs which are spherical,
triangular and flower shape with wavelength in the range of 420 to 430 nm, 395 to
495 nm and 293 to 300 nm respectively. XRD diffractogram of flower shaped indicated
face-centered cubic crystalline structure. The antimicrobial properties of Ag-NPs
against Staphylococcus aureus sp. and Escherichia coli sp. carried out by disc diffusion
method showed that flower shape Ag-NPs at pH 4 demonstrated highest inhibition
zones against Escherichia coli sp. For Staphylococcus Aureus sp., spherical Ag-NPs
demonstrated better result.
450 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018