merged

Levels of cytochrome c oxidase I (COI) divergence in Family Lutjanidae
using sequences available in GenBank and BOLD Systems
Muhamad Faizal Bin Talib
Supervisor: Dr Tun Nurul Aimi Mat Jaafar
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
DNA barcoding is a rapid growing molecular technique for species identification. This
technique using a short DNA sequence of mitochondrial DNA. Cytochrome C Oxidase
subunit I (COI) which is approximately 650 bp to discriminate individuals until specieslevel.
However, accuracy of public databases such as GenBank and BOLD as public
references for COI data and levels of CO1 devergence in family Lutjanidae are still
unknown. Here, a total of 72 sequences from 11 species of Family Lutjanidae has been
downloaded from public databases. The mean intraspecific K2P divergences were
1.1% among individuals within species and mean congeneric 6.1% within the genera.
There were two species have shown as deep intraspecific divergence of 4.3% and 3%
have been emphasized because is too high for same species. The accuracy of public
databases should be analysed for further study and that has been proven functional
of COI sequences as a tool for distinguish species.
497 | UMT UNDERGRADUATE RESEARCH DAY 2018