merged

Isolation of Photosynthetic Bacteria from Aquaculture Pond
Azri Bin Samad
Supervisor: Dr Shumpei Iehata
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture
The photosynthetic bacteria are characterized by their ability to grow in the absence
of air when exposed to light. Unlike the algae and higher plants, they do not produce
oxygen and require an external supply of an oxidizable substrate whose nature varies
with the species. Photosynthetic bacteria also can be used as food additive and can
control water quality in aquaculture pond. The objective of the study was to attempt
to isolate and identify photosynthetic bacteria from three different aquaculture pond
which is iSHARP (Integrated Shrimp Aquaculture Park), Akuatrop Setiu, and UMT pond
using enrichment culture technique. All experiments were conduct in autoclaved screw
cap test tube containing culture medium which was neutralized to pH 6.7 with NaOH.
Culture purity was examined microscopically and by streaking the culture on agar
plate. In vitro amplification of extracted 16S rDNA was done with universal primers
8F. The 16S rDNA partial sequences were blasted in GenBank. The isolate were
Comamonas aquatica, Acinetobacter gyllenbergii, Achromobacter xylosoxidans,
Pannonibacter phragmitetus, Pseudomonas mendocina, and Cloacibacterium
normanense and all species were Gram-negative bacterium. All the bacteria were not
photosynthetic bacteria but they may be helpful to aquaculture.
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561 | UMT UNDERGRADUATE RESEARCH DAY 2018