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Population Genetics of Two-Spined Yellow-Tail Stargazer (Uranoscopus<br />

Cognatus) From Malaysian Waters and Thailand Waters Using<br />

Mitochondrial DNA COI Gene<br />

Nuralif Fakhrullah bin Mohd Nur<br />

Supervisor: Dr. Tan Min Pau<br />

Bachelor of Applied Science (Fisheries)<br />

School of Fisheries and Aquaculture<br />

This study investigates the genetic diversity and population structure of the Twospined<br />

Yellow-tail Stargazer (Uranoscopus cognatus) using mitochondrial DNA. Three<br />

populations from Malaysian waters and Thailand waters consisting 53 samples were<br />

examined based on cyctochrome c oxidase subunit I gene (COI). A 506 base pair (bp)<br />

fragment of mitochondrial DNA COI gene was analyzed and a total of 17 polymorphic<br />

sites obtained resulting in 16 haplotypes. Molecular diversity indices revealed high<br />

level of haplotype diversity (h = 0.745) and low level of nucleotide diversity (π =<br />

0.003). Analysis of molecular variance (AMOVA) and pairwise F ST values show<br />

significant genetic structure among the populations with limited occurrence of gene<br />

flow. Results obtained provide crucial background information for further studies and<br />

proper management of the U. cognatus populations.<br />

629 | UMT UNDERGRADUATE RESEARCH DAY 2018

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