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Isolation and Identification of Hydrocarbon Degrading Bacteria from
Polychaete (Marphysa Moribidii)
Rozilawati Binti Mohamed Razali
Supervisor: Dr. Fazilah Binti Ariffin
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Hydrocarbon consumption has been rising significantly. Incidents such as accidental
spills caused severe contamination. It is crucial to eliminate hydrocarbon pollutant.
Bioremediation is a method which use microorganisms like bacteria to detoxify
pollutants. Bacteria that able to degrade hydrocarbon known as hydrocarbon
degrading bacteria. The objectives are to isolate and identify the bacteria from gut
fluid of Marphysa moribidii that can degrade diesel oil and their diesel oil degradation
cability. This study may able to discover bacteria with most efficiency in treating diesel
oil contaminants. Bacteria isolates were screened and enumerated in MSM with 1%
v/v of diesel and incubated in rotary shaker for 20 days. Percentage of degraded diesel
oil is determined using gravimetric analysis. Isolates; Bacillus cereus and
Staphylococcus kloosii able to degrade diesel oil with average percentage 52.29% and
39.24% respectively. However, T-test showed there is no significant difference
between these two species in degrading diesel oil.
372 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018