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Optimization of Sterilization Technique for Shoot Tissue Culture of
Cattleya Sp
Ellydia binti Chandra
Supervisor: Dr. Ma Nyuk Ling
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Micropropagation supports Cattleya sp. orchids that have long generation time,
difficult to germinate and have low seeds production. This study is conducted to
optimize the sterilization techniques for shoot tissue culture of Cattleya. Shoots were
treated with 1% Clorox for 1, 3, 5, 7 and 10 mins, 1% Clorox for 1, 3 and 5 mins with
0.2% fungicides (Fuji-One and Moncut) for 5 mins and 0.5% Clorox for 1, 3 and 5
mins with 0.2% Moncut for 5 mins. Treatment with only 1% Clorox could not control
fungal contamination while long exposure to Clorox leads to less browning but high
explants contamination. Treatment with 1% Clorox for 3 mins with 0.2% Moncut yields
80% of explants survival rate. Further studies should be focusing on micropropagation
of different plant hormone to induce callus and shoot formation.
343 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018