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Bacterial Composition in Cloacal Mucus from Green Sea Turtles<br />

(Chelonia mydas) at Pulau Redang<br />

Fatin Nurain binti Norlizam<br />

Supervisor: Dr. Muhammad Hafiz bin Borkhanuddin<br />

Bachelor of Science (Marine Biology)<br />

School of Marine and Environmental Sciences<br />

Sea turtles are the ancient marine reptiles that have a very important role in our<br />

marine environment. There are many causes of the declination of their population<br />

including diseases. Diseases come from three factors which are toxicological,<br />

physiologic and microbial etiologies. Microbial organisms have been attributed to lower<br />

hatching success of the juveniles, which may leads to the population decrease. The<br />

objectives of this study are; 1) to determine the composition of bacteria and 2) to<br />

identify the types of bacteria that present in the cloacal mucus of green sea turtles.<br />

The results obtained reveals the present of six different bacteria strains. Four of them<br />

were successfully identified through 16s rRNA, which are Ochrobactrum intermedium<br />

(Strain T1), Pseudochrobactrum lubricantis (Strain T2), Klebsiella variicola (Strain T4)<br />

and Shewanella upenei (Strain T6). O. intermedium shows the highest colony count<br />

in all samples and the least is the unidentified species, Strain T3.<br />

1816 | UMT UNDERGRADUATE RESEARCH DAY 2018

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