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The Effect of Namalycastis sp. Extract on Mcf-7 Cell Growth and
Proliferation
Yan Chong Seng
Supervisor: Dr. Maziah Binti Mohd Ghazaly
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Breast cancer is a challenging medical issue due to its multi-drug resistant behaviour.
A marine polychaete, Namalycastis sp., may have a potential therapeutic use on
treating cancer. There are insufficient data shown in using polychaete as potential
treatment for anticancer. Thus, this study was aimed to investigate the effects on
growth and cell proliferation against the human adenocarcinoma breast cancer cell
line, MCF-7 cells. The cytotoxic effect of Namalycastis sp. extract showed the IC50 at
6.875mg/mL after 24 hours of treatment detected by MTT assay. Following treatment,
MCF-7 cell death mechanisms was found to be activated through caspase 3/7 that
leads to cell apoptosis process detected by caspase 3/7 assay. Furthermore, the
overproduction of reactive oxygen species in MCF-7 was attenuated (66.52%)
following treatment compared to control (74.58%). Thus, Namalycastis sp. may have
a promising therapeutic potential for treating oxidative-stress induced cancer diseases.
Keywords: Namalycastis sp., cancer, reactive oxygen species, apoptosis, cellular
signalling mechanism
377 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018