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Preliminarily Study on Methanolic Crude Extracts of Sonneratia sp. and<br />

Antioxidant Activity<br />

Sahira Afifah Binti Mohd Razipi<br />

Supervisor: Dr. Noor Wini binti Mazlan<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Sciences<br />

Universiti Malaysia Terengganu<br />

The development of clean, non-toxic and eco-friendly methods are gaining importance<br />

in exploring the bioactive compounds from the plant extracts. The main aim of this<br />

study was to preliminarily screen the secondary metabolites from the leaves, fruits,<br />

stems and twigs of Sonneratia sp. which have been collected at Setiu Wetlands,<br />

Terengganu for its potential antioxidants activity. The extraction was done using cold<br />

extraction to obtain methanolic crude extract. The highest methanolic crude extracts<br />

were the leaves (18.43%) followed by the twigs (14.91%), fruits (14.72%) and stems<br />

(11.32%) from Sonneratia sp. The secondary metabolites were identified by using ID<br />

and 2D nuclear magnetic resonance (NMR) including COSY and HMBC coupled with<br />

multivariate analysis (SIMCA P +14). The most potent antioxidant using DPPH<br />

scavenging activity showed that the crude extract from the twigs of Sonneratia sp.<br />

with inhibition concentration at 50% (IC50) value of 0.09 mg/mL.<br />

1971 | UMT UNDERGRADUATE RESEARCH DAY 2018

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