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The Effects of Namalycasti S sp. Lipid Extract on High Glucose- Induced
Oxidative Stress in 3t3 Fibroblast Cell
Siti Nur Sharmila Binti Abdullah
Supervisor: Dr. Maziah Binti Ghazaly
Bachelor of Science (Biological Sciences)
School Of Fundamental Science
Hyperglycemia is defined by having high level of glucose in blood. Additionally,
Namalycastis sp. was chosen due to its ability to undergo fast and complete selfregeneration.
However, limited research on it lipid function. 3T3 fibroblast cells were
stimulated by high glucose and treated with various concentrations (0-1600 ug/mL)
of Namalaycastis sp. lipid extract for 24 hours. In this study, Namalycastis sp. lipid
extract was able to reduced 3T3 cells viability in normal glucose, however it may not
have any protective effect on cells against high glucose-induced damage detected by
MTT assay. The lipid extract was able to trigger cells proliferation in normal glucose
at 6.25ug/mL within 15 hours detected by migration assay. A trend of reduction on
reactive oxygen species level found in cells following treatment under normal glucose
concentration. Conclusively, this study found that Namalycastis sp. lipid extract may
have therapeutic potential in cells regeneration under normal glucose condition.
Keyword: Namalycastis sp., 3T3 fibroblast cell, high glucose, reactive oxygen species
375 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018