13.05.2018 Views

merged

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Bioluminescent Bacteria as a Potential Biosensor for the Detection of<br />

Heavy Metal Contamination<br />

Nurfatin Afiqah binti Sidek<br />

Supervisor: Assoc. Prof. Dr. Muhd Danish Daniel bin Abdullah<br />

Bachelor of Applied Science (Fisheries)<br />

School of Aquaculture and Fisheries Sciences<br />

Bioluminescent bacteria (BLB) are one of biosensors that could be used as an indicator<br />

to environmental pollutants. This scientific study determined the responses of BLB<br />

isolated from squids towards some heavy metals that are commonly found in the<br />

aquatic environment (Cadmium (Cd), Copper (Cu), Zinc (Zn), Iron (Fe)). Identification<br />

using VITEK shows that, 93% probabilities of the bacteria were Photobacterium<br />

damselae. The BLB were applied in assessing the toxicity of those heavy metals in<br />

different concentration ranging from 0.1 to 2.0 ppm. As a result, the BLB light intensity<br />

showed a declination once they were introduced to the toxicant. Thus, it concludes<br />

that, the light intensity emitted by the BLB is inversely proportional to toxicant<br />

concentration. The results also suggested that Photobacterium isolated in this study<br />

can be used as biosensor for the detection of heavy metals contamination. Further<br />

study should be carried out in the future involving field experiments.<br />

630 | UMT UNDERGRADUATE RESEARCH DAY 2018

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!