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Fatty Acid Profiling of Polychaetes and their Potential against Tumor Cell<br />

Thibaashiny A/P Panneer Selvom<br />

Supervisor: Dr. Tuan Nurul Sabiqah binti Tuan Anuar<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Sciences<br />

Universiti Malaysia Terengganu<br />

A new species of Namalycastis polychaete collected from mangrove area in Setiu<br />

Wetlands, Terengganu was analysed to extract fatty acid content using Bligh and Dyer<br />

method and later identified using Gas Chromatography Flame Ionization Detector.<br />

MTT analysis was conducted to determine inhibition level of HeLa cell using different<br />

concentration of polychaete lipid. It was found that fatty acid content in the worm<br />

showed more saturated fatty acids (SFA: 44.79%) and monounsaturated fatty acids<br />

(MUFA: 34.55%) followed by polyunsaturated fatty acids (PUFA: 20.64%). This may<br />

due to type of food intake of the worm surrounding area. In anti-tumor analysis, the<br />

percentage of HeLa cell viability decreases against increasing concentrations and<br />

shows inhibition. MUFA and PUFA might inhibit the HeLa cell due to the oleic acid,<br />

dihomogammalinolenic acid (DGLA), eiocosapentanoic acid (EPA) and docosahexanoic<br />

acid (DHA). Overall, estuarine polychaete are important in nutritional value and cancer<br />

cell reduction.<br />

1975 | UMT UNDERGRADUATE RESEARCH DAY 2018

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