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Molecular Detection of White Spot Syndrome Virus in the Ectoderm of
Marphysa Moribidii Collected From Morib, Selangor
Nur Syuhada binti Zakaria
Supervisor: Dr. Siti Nor Khadijah binti Addis
Bachelor of Science (Biological Science)
School of Fundamental Science
Malaysia are well-known for being a significant area for shrimp culture. However, the
emergence of White spot disease (WSD), which is a significant viral shrimp pathogen
caused by a White spot syndrome virus (WSSV) is considered the most challenging
problems for this industry. WSSV had caused many farms to close down due to mass
mortalities of shrimp. This virus can infect a wide range of host. Here, we investigated
whether the polychaete, Marphysa moribidii is positive with WSSV and hence could be
a positive carrier for this virus through the nested polymerase chain reaction. Our
result shows that out of five randomly collected polychaetes, 0 (0%) were found to
be positive by primary PCR and only 1 (20%) were found to be positive by secondary
PCR. The data indicate that the polychaetes might act as a WSSV reservoir to shrimp
aquaculture. Our findings are the first detection of WSSV from M. moribidii collected
from Morib, Selangor, Malaysia.
340 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018