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In Vitro Response of Isolated Fungi from Mangrove in Morib to Salt Stress
Nurin Sahira Binti Muda
Supervisor: Dr. Nurul Aliaa Bt Idris
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Salinization of soil is a severe problem that affects the establishment, growth and
development of plant. Salt stress has become a major threat to plant growth that lead
to huge loses in productivity of plants especially in agricultural purposes. This study
aim to isolate and identify the mycorrhizal fungi associating with mangrove plant and
to determine the tolerance of isolated mycorrhizal fungi under in vitro salt stress.
Three fungal species which are Penicillium pinophilum, Aspergillus fumigatus,
Aspergillus niger were isolated and identified. The isolated fungi were screened for
salt stress tolerance at four different salt (NaCl) concentrations (0, 0.05, 0.10, 0.15
M) in Potato Dextrose Agar (PDA). Tolerance was measured by diameter growth rate
and dried biomass. The results showed that the three isolated fungal species were
positively affected by the NaCl treatments as the growth rates of isolated fungi
increased with the increasing NaCl concentrations (0, 0.05, 0.10, 0.15 M).
365 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018