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Optimization of Silver Nanoparticle Reducing Agent from Marphysa
Moribidii Extract and Its Antimicrobial Activities
Mohammad Asyraf Adhwa bin Masimen
Supervisor: Assoc. Prof Dr. Wan Iryani Wan Ismail
Co-Supervisor: Dr. Noor Aniza Harun
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Silver nanoparticles (AgNPs) synthesis using local species of polychaete, Marphysa
moribidii as a reducing agent is relatively new. However, the optimum time for AgNPs
synthesis using this organism as reducing agent and its efficiency as antimicrobial
agent is still unknown. Through this experiment, the synthesis time is relatively fast
as the reduction process starts as soon as the silver nitrate solution is mixed with the
polychaete crude extract. Optimum time for the synthesis is between four to five days,
based on surface Plasmon resonance (SPR) band exhibited by AgNPs showing a single
sharp peak formation approximately at 401 – 405 nm. The aqueous AgNPs can also
be converted to solid form using freeze-drying technique. From scanning and
transmission electron microscopy (SEM and TEM) analysis, AgNPs have spherical
shape. Minimum inhibitory concentration and spread plate technique assessment on
bacteria and yeast indicated that AgNPs are able to function as antimicrobial agent.
379 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018