merged

Sub-acute toxicity (renal function test, lipid profile test and blood glucose
test) of black seed (Nigella sativa) and honey mixture.
A’ishah Binti Sahiron
Supervisor: Dr. Hayati Binti Mohd Yusof
Bachelor of Food Science (Food Service and Nutrition)
School of Food Science and Technology
To date, there are several researches investigated the beneficial effect of black seed and
honey however only few studies on the mixture. Previously, a research about the toxicity
effect of the black seed and honey mixture was performed, but limited to liver function
test and the histopathology analysis. Therefore, the aim of this study is evaluate the subacute
toxicity of the mixture on renal function test, lipid profile test and blood glucose
test. Toxicity study will be conducted according to OECD guidelines 420 Thirty male
Sprague Dawley rats (120-300 g) will be and will be treated with different dosage of
black seed and honey mixture (100, 500, 1000 and 2000 mg/kg, respectively) for 14
days. It is anticipated that no toxicity of black seed and honey mixture on the renal
function test. Furthermore, effect of the high dosage on lipid profile and glucose level will
also be documented.
1136 | UMT UNDERGRADUATE RESEARCH DAY