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Molecular Detection of White Spot Syndrome Virus in Diopatra Claparedii
Collected From Morib, Selangor
Azwin Farhin binti Yaakob
Supervisor: Dr. Siti Nor Khadijah Addis
Bachelor of Science (Biological Science)
School of Fundamental Science
White spot syndrome virus (WSSV) outbreaks occurred among aquaculture areas by
causing great economic loss and high mortality rate among shrimp species. The
polychaete worm plays a role in epizootiology of WSSV as a passive vector in the
transmission and infection of the virus. Less investigation has used polychaete as the
model for WSSV detection due to the virus competency to retain its infectivity.
Diopatra claparedii collected from Pantai Kelanang, Morib, Selangor was used as the
non-crustacean model in this study. The objective of this project was to detect WSSV
presence in D. claparedii using nested polymerase chain reaction (PCR) with specific
primers. PCR analysis produced no DNA amplicons of expected size. WSSV failed to
reside in D. claparedii, indicate that the polychaete may not be WSSV passive vector.
In conclusion, this study suggests that there is no occurrence of WSSV in D. claparedii.
Keywords: Polychaete, Diopatra claparedii, White Spot Syndrome Virus, PCR, Morib
341 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018