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Evaluation of Cytotoxicity Activities of Gentadexa on Acanthamoeba
Trophozoite
Nur A’fiefah Binti Mohd Zulkeffli
Supervisor: Dr Fatimah Binti Hashim
Co-Supervisor: Dr Muhammad Fairus Bin Noor Hashim
Bachelor of Science (Biological Sciences)
School of Fundamental Science
In this study, Acanthamoeba was treated with Colircusi Gentadexa (CG) in order to
study the cytotoxicity effects of CG on Acanthamoeba based on MTT assays, light
microscope for morphological changes and acridine orange and propidium iodide
(AO/PI) staining for determination of cell death. The IC50 value obtained was in the
moderate range which is 20.3%. Morphological changes of treated Acanthamoeba for
24 hours were observed under light microscope showed the inhibition of acanthapodia
on surface of the cells. CG-treated Acanthamoeba was also stained with AO/PI staining
solutions indicated the presence of red Acanthamoeba cells, a necrotic type of cells.
This was resulted from the leakage of Acanthamoeba cell membrane after exposed to
CG at its IC50 value. It is shown that CG is able to kill trophozoite of Acanthamoeba
cells with moderate IC50 value. Unfortunately, necrosis mode of cell death is not
preferable type of cell death for Acanthamoeba to be killed.
335 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018