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Study of Reactive Oxygen Species (ROS) in Gonad of Sea Urchin at Pulau<br />

Bidong<br />

Ahmad Safuan Bin Sallehuddin<br />

Supervisor: Dr. Siti Nurtahirah Binti Ja’afar<br />

Bachelor of Sciences (Marine Biology)<br />

School of Marine and Environmental Sciences<br />

Tropical sea urchin are vulnerable to the global warming and anthropogenic activity<br />

which induce the production of reactive oxygen species (ROS) in the organism. Level<br />

of ROS will induce tissues damage including gonad. Gonad obtained from sea urchins<br />

used to detect the oxidative stress induced by this targeted organism in marine<br />

ecosystem. This study was conducted to investigate the level of Gluthathione S-<br />

transferase (GST) and Catalase (CAT) in gonads of sea urchins from three different<br />

sites in Pulau Bidong. Level of CAT and GST activities were measured by using<br />

spectrophotometer with different wavelength of 240nm and 340nm respectively.<br />

Results shown the level of GST were significantly highest (p

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