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Qualitative Phytochemical Screening and Antioxidant Activities from Three
Different Citrus Leaves (Rutaceae)
Nur Irdeena Binti Zulkornain
Supervisor: Pn Nurul Huda Binti Abdul Wahab
Bachelor of Science (Chemical Sciences)
School Of Fundamental Sciences
Citrus has long been considered as a food and curative plant. Leaves of Citrus
aurantifolia, Citrus hystrix and Citrus microcarpa were selected to investigate their
phytochemical contents and antioxidant activities. The leaves extraction of those
samples were carried out using three different polarities of solvents (hexane, ethyl
acetate, and methanol). Phytochemical screening disclosed that the leaves of C.
aurantifolia have positive results of alkaloids, saponins, flavonoids, reducing sugar and
carbohydrates only in hexane and ethyl acetate crude extracts. Meanwhile, C. hystrix
leaves contained with alkaloids, triterpenoids, saponins, reducing sugar,
carbohydrates, phenolic and tannins in all crude extracts. However, flavonoids and
glycosides only existed in hexane and methanol crude extracts. From C. microcarpa
leaves, indicated that the presence of alkaloids, triterpenoids, saponins, reducing
sugar, carbohydrates, phenolic and tannins in hexane and ethyl acetate extracts.
Additionally, the non-enzymatic antioxidants assays were conducted and showed the
promosing results of α-tocopherol and carotenoids contains.
468 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018