merged

Levels of cytochrome oxidase I (COI) divergence in Family Lethrinidae
using sequences available in GenBank and BOLD Systems
Nurul Hidayatul Adha Binti Tajri
Supervisor: Dr Tun Nurul Aimi Mat Jaafar
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
DNA barcoding is useful in solving taxonomic ambiguities by using cytochrome oxidase
I (COI) gene. The levels of COI divergence of family Lethrinidae and the accuracy of
COI sequences available in public databases were analysed. A total of 200 sequences
of 20 species from family Lethrinidae has been downloaded from these public
databases. Two COI sequences showed ambiguous sites and therefore were excluded
for further analysis. A total of 27 sequences has been detected as potential
misidentification as these sequences did not group with their own taxa. The mean
intraspecific K2P divergences were 7.82% among individuals within species and
49.63% within genera. There were 11 species that had shown deep divergences
among individuals with maximum intraspecific divergence of 19.2%, 18.7%, 18.3%,
18.2%, 14.8%, 12.2%, 11.5%, 10.7%, 9.6%, 9.3% and 4.5% respectively. The public
databases is important as reliable references and COI has been proved can
discriminate species efficiently.
523 | UMT UNDERGRADUATE RESEARCH DAY 2018