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Genetic Diversity of Tumpnose Travelly, Carangoides Hedlandensis in
Malaysia Using Mitochondrial DNA Cytochrome C Oxidation Subunit 1
(CO1)
Sangary Ravindran
Supervisor: Dr Tun Nurul Aimi Bt. Mat Jaafar
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture
Fisheries resources have limited data on the genetic basis of stock structure.
Carangoides hedlandensis is one of the species that has limited data due to higher
possibility of misidentification. Thirty nine samples of C.hedlandensis were collected
from 10 different locations in Malaysia. DNA was extracted from all the samples and
cytochrome c oxidation subunit 1 (CO1) gene for the DNA undergoes PCR amplification
process. All the sequences were analysed to determine genetic diversity and
population structure of C.hedlandensis. Results showed that average haplotype
(h=0.9973) and nucleotide (ᴨ=0.01964) diversities were indicated the genetic
diversity of C.hedlandensis is higher in Malaysia. The Fst value of this population was
0.23823 revealed that moderate genetic structure. The structure of neighbour –
joining phylogenetic tree of C.hedlandensis formed eight small monophyletic cluster
and two paraphyletic groups. Lack of genetic structuring among the samples was
depicted by AMOVA analysis. In conclusion, it’s refer all sample in one population.
564 | UMT UNDERGRADUATE RESEARCH DAY 2018