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Biosynthesis of Silver Nanoparticles (AgNPs) by Tomato Extract and Its
Antimicrobial Activity
Najihah binti Nasran
Supervisor: Dr. Hanis binti Mohd Yusoff
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Silver nanoparticles (AgNPs) have been studied extensively due to their unique
properties and wide range of applications in various fields, which differ significantly
from bulk materials. In this study, AgNPs was synthesis from aqueous extract of
tomato (Solanum lycopersicum) with different concentrations of silver nitrate. The
formation and morphology of synthesized AgNPs have been characterized by using
UV-Visible spectroscopy (UV-Vis) and Scanning Electron Microscopy (SEM). UV-Vis
spectroscopy analysis showed a peak at 420 nm which indicated AgNPs formation and
SEM images showed spherical AgNPs. Furthermore, disc diffusion method was used
to evaluate the antimicrobial activity of AgNPs. The antimicrobial activity of
synthesized AgNPs was tested with Gram-positive (Staphylococcus aureus) and Gramnegative
(Escherichia coli) bacterium. AgNPs synthesized from the tomato extract with
1x10 -1 M and 1x10 -3 M of substrate have better antimicrobial activities against S.aureus
(17 mm of zone inhibition) and E.coli (16 mm of zone inhibition) respectively.
423 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018