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The Morphological Characteristics of Hemocytes and Gluthatione S-<br />

Transferase Activities in Meretrix meretrix<br />

Revati Ganasan<br />

Supervisor: Dr. Siti Nurtahirah bt Ja’afar<br />

Bachelor of Sciences (Marine Biology)<br />

School of Marine Science and Environment<br />

Haemocytes of Asiatic Hard Clam, M. meretrix has been characterized by its<br />

morphology. The present study indicated that three main cell types were found in<br />

M.meretrix; Granular haemocytes, hyalinocytes and lymphoid cells. The study was<br />

carried out, both by using light microscope and scanning electron microscope (SEM).<br />

The GST analysis was also done using the hemolymph of M.meretrix and was<br />

conducted using UV-vis spectrophotometer. However, GST activity tested on two sites<br />

showed that Site 1 was much higher compared to Site 2. The granular haemocytes<br />

had dense nucleus and low ratio of nucleus to cytoplasm, whereas, both hyalinocytes<br />

and lymphoid cells had less-dense nucleus and high nucleus to cytoplasm ratio. In<br />

addition, SEM images of haemocytes showed an irregular structure and granulated<br />

surface. The mechanism of action for GST indicates the oxidative stress level in the<br />

clams of two sites but there was no significant difference between them as (p>0.05).<br />

1842 | UMT UNDERGRADUATE RESEARCH DAY 2018

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