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Comparison of Two Housekeeping Genes (Cyclophilin And
Alpha Tubulin) Of Chlorella Vulgaris Grown Under
Nitrogen Limited Condition
Siti Nurul Amira Binti Noor Azman
Supervisor: Dr. Malinna Binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Real time Polymerase Chain Reaction (PCR) is one of the methods to study gene
expression between samples and experimental conditions. Thus, the comparison
between two housekeeping genes, cyclophilin and alpha tubulin were observed to
evaluate the most stable gene expression for the use as reference gene in real time
PCR. Cells of C. vulgaris were cultured in F/2 media with 100% control and 25%
treatment of sodium nitrate. Cells were collected at 0, 7, 14, 21 and 28 days after
treatment, proceeded with RNA extraction, cDNA synthesis and analysed by real-time
PCR. The expression data were obtained from real time PCR threshold (Ct) values.
From the results, cyclophilin had stable expression for both control and treatment
where the reading recorded were 28.04 ± 1.37 and 29.17 ± 1.22, respectively.
Cyclophilin was the most stable gene and appropriate for the normalization of gene
expression studies for real time PCR.
353 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018