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EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES
FROM VITEX ROTUNDIFOLIA FRUITS EXTRACT
SITI KHODIJAH BINTI KOSIM
Supervisor: Dr. Suvik A/L Assaw
Co. Supervisor: Dr. Noor Wini binti Mazlan
Bachelor of Science (Marine Biology)
School of Marine and Environmental Sciences
Marine environment provides a valuable platform for discovery of new
biologically active compounds. In this study, coastal medicinal plant, Vitex
rotundifolia was evaluated for its antibacterial and antioxidant properties.
Methanolic crude extract from fruits of V.rotundifolia was fractionated using
vacuum liquid chromatography (VLC). The selected fractions were evaluated
for antibacterial and antioxidant using well-diffusion and DPPH free radical
scavenging assays respectively. Results revealed in antibacterial assays, all
fractions give inhibition to Bacillus subtilis, meanwhile fractions 2, 7, 10, 16, 18,
and 24 give inhibition on Staphylococcus epidermidis. No antibacterial activities
were found on other bacterial tested. Antioxidant results demonstrated 50%
inhibition concentration (IC50) values for fraction 7, 10, 16, 18, and 24 were
0.9, 0.88, 0.625, 0.375 and 0.375 mg/mL respectively. However, fraction 2 and
6 were having IC50 more than 1mg/mL. As conclusion, V.rotundifolia fruits has
antibacterial and antioxidant activities but it’s depend on solvent system used
to fractionate the targeted compound.
1784 | UMT UNDERGRADUATE RESEARCH DAY 2018