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MOLECULAR IMPRINTED POLYMER FOR DOMOIC ACID DETECTION
Han Zhen Yang
Supervisor: Dr. Hafiza binti Mohamed Zuki
Bachelor of Science (Analytical and Environmental Chemistry)
School of Marine and Environmental Sciences
Universiti Malaysia Terengganu
Domoic acid imprinted polymers (MIPs) were successfully synthesized by bulk
polymerization method using 2-hydroxyethyl methacrylate (HEMA) as a functional
monomer and ethyleneglycol dimethacrylate (EGDMA) as a cross-linker. Nonimprinted
polymers (NIPs) were also synthesized using similar procedures but without
the addition of template molecules (DA). The presence of DA template in MIPs and
the absence of DA template in NIPs were proven by Fourier-Transform Infrared (FT-
IR) Spectroscopy. All MIPs analyses were done using UV-Vis spectrophotometer.
Binding efficiencies of MIPs synthesized to domoic acid were determined using batch
rebinding experiments where the optimum mass and time obtained were 20 mg and
60 minutes respectively. Meanwhile, the limit of detection (LOD) and limit of
quantification (LOQ) calculated were 0.26 ppm and 0.43 ppm respectively.
1942 | UMT UNDERGRADUATE RESEARCH DAY 2018