merged

Probiotics isolation from striped catfish (Pangasius hypophthalmus
sauvage 1878) gut
Fautasha binti Zakaria
Supervisor: Dr Shumpei Iehata
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Catfish from the family Pangasiidae especially Pangasius hypophthalmus (Sauvage
1878) is a species for aquaculture because omnivorous feeding habits, rapid growth
and good market. Striped catfish have immense economic important in many country
of Southeast Asia including Malaysia. Striped catfish has been expended rapidly and
causes the disease outbreak such as bacillary necrosis of Pangasius (BNP). Many
farmer willing to invest in chemical such as antibiotic that have been banned for
aquaculture used to minimize the disease. Probiotics is used to control the disease
outbreak in aquaculture. The aim of this study is to isolate probiotics candidate from
gut using pour method. Isolation of the probiotics candidates ware used MRS agar
with 2% Calcium Carbonate (CaCO3) and chosen colony with clear zone. Isolates were
tested in vitro antipathogenicity against Vibrio parahaemolyticus using well-diffusion
method. Then, isolates with positive antipathogenicity were further identified by
polymerase chain reaction (PCR) and sequencing. From 9 initial isolated bacteria, 4
were show the positive results. Sequence for these isolates are now in progress.
485 | UMT UNDERGRADUATE RESEARCH DAY 2018