merged

Inhibition Of Histamine Producing Bacteria, Acinetobacter Iwoffii By Using
Stingless Bee Honey, Tetragonula Zucci, Tetragonula Fuscobalteata And
Heterotrigona Itama
Haliza Hani binti Kamiruddin
Supervisor: Dr. Nurul Ulfah Karim
Bachelor of Applied Science (Fisheries)
School of Fisheries and Aquaculture Sciences
This study emphasize on isolation of lactic acid bacteria (LAB) and inhibition of
histamine producing bacteria (HPB) in Indian mackerel by using stingless bee honey
with concentrations of 50, 80 and 100%. The isolation of LAB from stingless bee honey
conducted using MRS agar with 0.8% CaCO3 while isolation of HPB were isolated from
Indian mackerel using Niven agar. All LAB and HPB colonies were purified with a
streaking method followed by morphological and biochemical test. All isolates were
further characterized phenotypically using BBL Crystal kit. Stingless bee honey at
concentrations of 50, 80 and 100% were tested for antibacterial activities, using disc
diffusion assay against HPB, Acinetobacter iwoffii. As the result, stingless bee honey
(at 50, 80 and 100% concentration) is resistant to Acinetobacter iwoffii. The LAB from
this study was unable to be isolated. The antibacterial properties of stingless bee
honeys containing hydrogen peroxide need to be validated.
586 | UMT UNDERGRADUATE RESEARCH DAY 2018