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Genetic variation in Pangasius nasutus broodstock from two different<br />

populations using internal transcribed spacer (its) marker<br />

Muhammad Faiz Bin Ali<br />

Supervisor: Dr Shahreza Bin Md. Sheriff<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

This study was conducted to determine the genetic variation in Pangasius nasutus<br />

broodstock taken from two different populations, Maran and Temerloh, Pahang<br />

respectively using Internal Transcribed Spacer (ITS) marker. A total of 20<br />

broodstock were collected and subjected to DNA extraction. Universal ITS primer<br />

were used for the PCR amplification and the fragments were sequenced. ITS gene<br />

sequence with 550bp were obtained from each sample and being examined. The<br />

samples are sending to FirstBase Company. The data was analysed by using MEGA<br />

V7.0 software. The ITS gene have been successfully amplified. The purpose of this<br />

project is to find genetic variation of a species in two different populations. There<br />

should be a variation in the species between two different populations. This project<br />

will discuss the result get from the company.<br />

503 | UMT UNDERGRADUATE RESEARCH DAY 2018

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