merged

Genetic Variation of Hedgehog Seahorse, Hippocampus Spinosissimus
Broodstock from Johor Population Using Mitochondrial DNA Cytochrome B
(cyt b)
Muhammad Syafiq Aiman bin Mohd Nasir
Supervisor: Dr Shahreza bin Md Sheriff
Bachelor of Applied Science (Fisheries)
School of Fisheries and Aquaculture Sciences
The Hedgehog Seahorse, Hippocampus spinosissimus is one of the seahorses listed
as endangered species in the IUCN Red List due to several factors such as
overexploitation, habitat loss, and bycatch. Genetic study is a suitable method to
assess the genetic variation among the individual species of the seahorse. A total of
22 H. spinosissimus were collected from Sungai Pulai and genetic analysis is carried
out using the mitochondrial DNA Cytochrome B and the partial Cytochrome B gene
was successfully amplified using PCR. The variation is determined by phylogenetic,
haplotype and nucleotide studies. The finding shows a variation between individual in
the population and this paper discuss the variation that occurred among the H.
spinosissimus population. The finding from the study could be used to assist the
propagation of the species in the future.
608 | UMT UNDERGRADUATE RESEARCH DAY 2018