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Study of Phytochemical Properties and Antioxidant Activity of Gracilaria sp
Nursyamen Izzaty Binti Azmi
Supervisor: Dr. Asnuzilawati Binti Asari
Bachelor of Sience (Chemical Sciences)
School of Fundamental Sciences
Gracilaria species or known as agar-agar red seaweeds are belonging to phylum
Rhodophyta and family Gracilariaceace. These species have been reported to posses
various biological activities including antiprotozoal, antiviral, antifungal, anti-bacterial,
cardiovascular effects and cytotoxicity. The aim of this study is to determine
phytochemical properties and antioxidant activities on Gracilaria sp. In this study,
Gracilaria sp was collected from Johor and was extracted by using two different solvent
with different polarities which are ethyl acetetate and methanol. Then, the extract was
evaluated for antioxidant activities by using two methods, namely (a) free radical
scavenging assay with DPPH and non-superoxide, (b) non-enzymatic antioxidant
assays which is to determine caratenoids and α- tocopherols. The phytochemical test
revealed that, the crude extracts of ethyl acetate contain alkaloid, reducing sugar,
steroid, and terpenoid. Meanwhile, for methanol crude extract, it contains saponin,
alkaloid, and terpenoid. The results obtained are important for identification of their
bioactive principles.
446 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018