merged

Development of Ninhydrin as a Sensing Reagent for Domoic Acid Detection
Using Enzyme-Linked Immunosorbent Assay (ELISA) method.
Nur Rahayu Wani binti Kassim
Supervisor: Dr. Hafiza binti Mohamed Zuki
Bachelor of Science (Analytical and Environmental Chemistry)
School of Marine and Environmental Science
Universiti Malaysia Terengganu
In this study, ninhydrin was developed as a sensing reagent for domoic acid (DA)
using Enzyme-linked Immunosorbent Assay (ELISA) method since ninhydrin can
detect a presence of DA in a complex mixture. Reaction of Anti-Mouse IgG (H&L)
Alkaline Phosphatase (AP) conjugate enzyme with p-Nitrophenyl phosphate (p-NPP)
substrate shows a positive reaction of ninhydrin with DA by producing a yellow colored
product from a colorless solution. The changes of color obtained were detected by
using microplate spectrophotometer. The influence of solvent used for dilution and
different types of buffer was optimized. The optimum solvent used obtained was
carbonate buffer solution. The optimum coating buffer and blocking buffer obtained
were sodium carbonate buffer and a mixture of 5% Bovine Serum Albumin (BSA) with
0.1% Tween-20 (T-20) in Phosphate Buffered Saline (PBS) respectively. 1 mg/L of
ninhydrin and DA were the optimum concentration obtained since they give the
highest absorbance reading of 0.4825 and 0.353, respectively.
1967 | UMT UNDERGRADUATE RESEARCH DAY 2018