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Chemical Investigation of Geniotrigona Thoracica 'S Propolis
From Dungun, Malaysia
Nursyaza Syazwani Binti Yasjar
Supervisor: Dr. Ahmad Nazif bin Aziz
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Propolis has many biological and pharmacological properties, which are related to its
chemical composition. In this study, the propolis produced by Geniotrigona thoracica
from Dungun, Terengganu was investigated for its chemical constituents. The propolis
was extracted by using four solvents which are methanol, hexane, dichloromethane
and ethyl acetate. The phytochemical screening showed the presence of flavonoids,
terpenoids, glycosides, alkaloids, steroids phenols and tannins. The hexane, methanol
and dichloromethane extracts showed mild cytotoxicity against MCF7- breast cancer
cell lines with IC50 values of 40 µg/mL, 58 µg/mL, 34 µg/mL respectively while SKUT1-
fibroid cancer cell lines showed that mild cytotoxicity for hexane with IC50 value of 48
µg/mL. Dichloromethane extract was selected for isolation and purification of
compounds by using Thin Layer Chromatography and Column Chromatography. Two
compounds were isolated and analyzed by using Infrared, Ultraviolet, Nuclear
Magnetic Resonance and Mass Spectroscopy.
447 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018