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Bacteria Isolation from Water Sample of Seahorse Cultivation Tank Before<br />

and After Filtration<br />

Muhammad Luqman bin Puaadi<br />

Supervisor: Prof. Dr. Mohd. Effendy<br />

Bachelor of Applied Science (Fisheries)<br />

School of Fisheries and Aquaculture Sciences<br />

A study on water microbiology quality was conducted at seahorse nursery of Aquatrop<br />

Hatchery. Samples from the water supply before the filtration system at the reservoir<br />

and after the filtration system prior to the displacement into the tanks. Isolation was<br />

done using sterile transport swab which was then inoculated onto the MNA and EMB<br />

agar using the serial dilution and MPN method. Pure culture was made to identify the<br />

bacteria. Results showed that formation of metallic colonies on EMB agar from the<br />

sample of water before the filtration system. Pathogenic bacteria Escherichia coli was<br />

identified. The filtration system is successful in filtering pathogenic bacteria, but<br />

improvements can be made for a better microbial water quality.<br />

666 | UMT UNDERGRADUATE RESEARCH DAY 2018

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