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Optimization of Quechers Method for Octylphenol Residue in Bivalve
Nurul Natasha Abdullah
Supervisor: Assoc. Prof Dr. Marinah Mohd Ariffin
Co-Supervisor: Dr. Wan Mohd Afiq Wan Mohd Khalik
Bachelor of Science (Analytical and Environmental Chemistry)
School of Marine and Environmental Sciences
Universiti Malaysia Terengganu
Octylphenol is from non-ionic surfactant that use in production such as in detergent.
Its have been detected in biota, sediment and soil. Octylphenol can give effect towards
marine life such as reduction in the marine population. In this study, the quick, easy,
cheap, effective, rugged and save (QuEChERS) method was develop to ensure the
efficiency on determination of octylphenol followed by analysis High Performance
Liquid Chromatography with Fluorescence detection (HPLC-FLD). The linearity for
analysis of HPLC-FLD was (r 2 ≥0.9983). The limit of detection (LOD) and limit of
quantification (LOQ) at 0.05 and 0.15 were obtained. The optimization of QuEChERS
was using Design of Experimental where 30 standard orders were performed and the
data was interpreted by minitab software. There are four parameters were observed,
which are volume of extractant, speed of centrifuge, pH and time extraction. From the
experimental the optimal amount of volume extractant is 2.5ml, speed of centrifuge
at 4000rpm, pH sample at 10 and time of extraction is 12.
1966 | UMT UNDERGRADUATE RESEARCH DAY 2018